Part:BBa_K1587001

acyl-CoA thioesterase 2

Figure 1 Butyrate formation catalyzed by tesB

The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

We used BBa_K1587001 with other genes to produce butyrate in Escherichia coli (Figure 2)

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 542
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 145
  Illegal SapI.rc site found at 157